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To PCR amply the sequence
ATCTTCTACG...............AAGCTTGCGG
TAGAAGATGC...............TTCGAACGCC
the required primers are
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- ATCTTCTA and CGAACGCC
- ATCTTCTA and CCGCAAGC
- TAGAAGAT and CGAACGCC
- TAGAAGAT and CCGCAAGC
Correct Option: B
For sequencing we need complementary forward and reverse primer to bind with target sequence.
