-
Positional cloning approach exploits information
-
- On the location of the gene in the genome
- On the status of its expression
- About the position of promoter relative to MCS
- About the position of the restriction sites
Correct Option: A
Positional cloning is a method of gene identification in which a gene for a specific phenotype is identified only by its approximate chromosomal location (but not the function); this is known as the candidate region. Initially, the candidate region can be defined using techniques such as linkage analysis, and positional cloning is then used to narrow the candidate region until the gene and its mutations are found. Positional cloning typically involves the isolation of partially overlapping DNA segments from genomic libraries to progress along the chromosome toward a specific gene.
